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Ubiquitin-Like Protein ISG15 (ISG15) Antibody
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Catalogue No: abx026299
Price:
US$326.25
(Size: 80 µl)
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ISG15 is secreted from monocytes in response to type I interferons and causes natural killer (NK) cell proliferation and an augmentation of non-MCH (major histocompatibility complex) restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted also by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. The induction of terminal differentiation in human melanoma cells is associated with alterations in ISG15 expression. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog.
Research Articles on Ubiquitin-Like Protein ISG15 (ISG15)
| Target | Ubiquitin-Like Protein ISG15 (ISG15) |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 31-61 amino acids from the N-terminal region of human ISG15. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P05161 (UniProt, ExPASy) |
| UniProt Secondary AC | Q5SVA4, Q7Z2G2, Q96GF0 |
| UniProt Entry Name | ISG15_HUMAN |
| NCBI Accession | NP_005092.1 |
| KEGG | hsa:9636 |
| String | 9606.ENSP00000368699 |
| Molecular Weight | Calculated MW: 17.9 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |