Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A) Antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Target	Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IF/ICC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IF/ICC: 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 91-117 amino acids from human LC3.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9H492 (UniProt, ExPASy)
UniProt Secondary AC	E1P5P4, E1P5P5, Q9BXW5
UniProt Entry Name	MLP3A_HUMAN
Gene Symbol	MAP1LC3A
String	9606.ENSP00000363970
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat, Cow and Zebrafish LC3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Microtubule-Associated Proteins 1A/1B Light Chain 3A (MAP1LC3A)

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