Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK) Antibody

Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function. is constitutively associated with the cytoplasmic portions of the cd4 and cd8 surface receptors and plays a key role in t-cell antigen receptor (tcr)-linked signal transduction pathways. association of the tcr with a peptide antigen-bound mhc complex facilitates the interaction of cd4 and cd8 with mhc class ii and class i molecules, respectively, and thereby recruits the associated lck to the vicinity of the tcr/cd3 complex. lck then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (itams) in the cytoplasmic tails of the tcr gamma chains and cd3 subunits, initiating the tcr/cd3 signaling pathway. in addition, contributes to signaling by other receptor molecules. associates directly with the cytoplasmic tail of cd2, and upon engagement of the cd2 molecule, lck undergoes hyperphosphorylation and activation. also plays a role in the il2 receptor-linked signaling pathway that controls t-cell proliferative response. binding of il2 to its receptor results in increased activity of lck. is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-tcr and mature alpha beta tcr.

Target	Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified recombinant fragment of human Lck expressed in E. coli.
Isotype	IgG2a
Form	Liquid
Purification	Unpurified ascites.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P06239 (UniProt, ExPASy)
UniProt Entry Name	LCK_HUMAN
Gene Symbol	LCK
GeneID	3932
OMIM	153390
HGNC	6524
KEGG	hsa:3932
Ensembl	ENSG00000182866
String	9606.ENSP00000337825
Enzyme Commission Number	EC 2.7.10.2
Molecular Weight	56 kDa
Buffer	Ascitic fluid containing 0.03% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Proto-Oncogene Tyrosine-Protein Kinase LCK (LCK)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue