Macrophage Inflammatory Protein 1 Gamma (MIP1g) Antibody Pair
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Price:
US$1,754.50
(Size: 5 × 96 tests)
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Macrophage Inflammatory Protein 1 Gamma (MIP1g) Antibody Pair for use in Sandwich ELISA assay development. This antibody pair contains:
Component | 5 × 96 tests | 10 × 96 tests |
Capture Antibody | 200 µg | 400 µg |
Biotin-Conjugated Detection Antibody | 50 µg | 100 µg |
Standard | 2 µg | 10 µg |
Please note that quantities and concentrations may change between different batches.
It is recommended to use this antibody pair with
abx098958 Antibody Pair Support Kit (Sandwich Method).
Target |
Macrophage Inflammatory Protein 1 Gamma (MIP1g) |
Reactivity |
Rat |
Tested Applications |
ELISA |
Recommended dilutions |
Dilute the Capture Antibody 125-fold with Coating Buffer. Dilute the Biotin-Conjugated Detection Antibody 200-fold with Detection Antibody Diluent. Optimal dilutions/concentrations should be determined by the end user. |
Form |
Liquid (Capture Antibody and Detection Antibody) |
Reconstitution |
Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Buffer |
The Capture and Detection Antibody both contain 0.1% sodium azide. |
Standard Form |
Lyophilized |
Assay Type |
Sandwich |
Capture Antibody Conjugation |
Unconjugated |
Detection Antibody Conjugation |
Biotin |
Concentration |
Capture Antibody: 0.5 mg/ml Biotin-Conjugated Detection Antibody: 0.2 mg/ml |
Availability |
Please enquire. |
Note |
This product is for research use only. |
Directions for use |
Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.
Recommended Procedure: - Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 µl per well). Seal the plate and incubate at 4 °C overnight or at 37 °C for 2 hours
- Aspirate the wells and wash with Wash Buffer (350 µl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper.
- Block the plate with Blocking Buffer (200 µl per well) at 37 °C for 1.5 hours.
- Repeat the aspiration/wash process in Step 2.
- Add 100 µl of standards or sample into the appropriate wells. Cover with a plate sealer and incubate at 37 °C for 1 hour.
- Repeat the aspiration/wash process in Step 2.
- Add appropriately diluted Biotin-Conjugated Detection Antibody (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 1 hour.
- Repeat the aspiration/wash process in Step 2.
- Add appropriately diluted Streptavidin HRP (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
- Repeat the aspiration/wash process in Step 2.
- Add Substrate Solution (90 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 10-20 min. Keep the plate in the dark and avoid exposure to light.
- Add Stop Solution (50 µl per well). Tap the side of the plate to ensure thorough mixing.
- Measure the absorbance immediately using a microplate reader set at 450 nm.
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