Magnetic Beads-Based Bacteria Genomic DNA Kit

Magnetic Beads-Based Bacteria Genomic DNA Kit provides a fast, simple, and cost-effective method for isolating the genomic DNA from bacterial cells. Its unique buffer system will efficiently lyse cells and degrade protein, allowing for DNA to be easily bound by the surface of the magnetic beads. The Phenol extraction and ethanol precipitation are not required, and the high-quality genomic DNA is eluted in the Release Buffer. The genomic DNA purified with the Magnetic Beads-Based Bacteria Genomic DNA Kit is suitable for a variety of routine applications. The entire procedure can be completed within 15-20 minutes (using most magnetic separators) or the kit can be easily adapted to satisfy most automated Nucleic Acid purification systems.

Kit component	Volume
Magnetic Bead	2 ml
Lysis Buffer	30 ml
Wash Buffer	80 ml
Release Buffer	20 ml

Target	Magnetic Beads-Based Bacteria Genomic DNA Kit
Tested Applications	PCR, RT-PCR
Storage	Store at room temperature.
Buffer	Not applicable.
Material Required But Not Provided	Absolute Ethanol Magnetic separator 1.5 ml microcentrifuge tubes Water bath (65 °C)
Availability	Shipped within 10-15 working days.
Note	This product is for research use only.
Directions for use	Lysis Aliquot up to 300 µl of bacterial culture and 300 µl of Lysis Buffer to a 1.5 ml microcentrifuge tube and mix fully. Incubate at 65 °C for 5 minutes. Begin to pre-heat the Release Buffer to 65 °C during this period. Add 300 µl of Absolute Ethanol to the lysate and mix well. DNA Binding Add 20 µl of Magnetic Beads and mix fully by gentle shaking for 3 minutes. Place the tube in a magnetic separator for 30 seconds. If the solution becomes viscous, increase the separation time. Discard the solution. Wash Add 800 µl of Wash Buffer and mix well. Place the tube in a magnetic separator for 30 seconds. Discard the solution. Release Add 200 µl of pre-heated (65 °C) Wash Buffer and mix fully. Incubate at 65 °C for 3 minutes, shaking the tube vigorously each minute. Place the tube in a magnetic separator for 1 minute. Transfer only the clear fraction of the solution to a clean tube.

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