Human Mannose Binding Lectin (MBL) Protein (Active)

Mannose Binding Lectin (MBL) Protein (Active) is an active protein from Human.

Target	Mannose Binding Lectin (MBL)
Origin	Human
Tested Applications	WB, SDS-PAGE
Host	E. coli
Conjugation	Unconjugated
Form	Lyophilized
Purity	> 98%
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH₂O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P11226 (UniProt, ExPASy)
UniProt Entry Name	MBL2_HUMAN
KEGG	hsa:4153
String	9606.ENSP00000363079
Molecular Weight	Calculated MW: 43.0 kDa Observed MW (SDS-PAGE): 43 kDa
Sequence Fragment	Leu130-Ile248
Sequence	L GKQVGNKFFL TNGEIMTFEK VKALCVKFQA SVATPRNAAE NGAIQNLIKE EAFLGITDEK TEGQFVDLTG NRLTYTNWNE GEPNNAGSDE DCVLLLKNGQ WNDVPCSTSH LAVCEFPI
Tag	N-terminal His tag and GST tag
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity	Active
Biological Activity	Mannose Binding Lectin (MBL), also known as Mannose Binding Protein C (MBL2), is a calcium-dependent lectin involved in innate immune defense, which binds mannose, fucose and N-acetylglucosamine on different micro-organisms, therefore results in activation of the lectin pathway of the complement system. Mannose Associated Serine Protease 2 (MASP2) has been identified as an interactor of MBL2 by forming complexes with MBL2, triggering the activation of the complement system, thus a binding ELISA assay was conducted to detect the interaction of recombinant human MBL2 and MASP2. Briefly, MBL2 was diluted serially in 10 mM Tris-HCl, 1 M NaCl, 5 mM CaCl₂, and 0.05% Triton X-100 (pH 7.4). Duplicate samples of 100 µl were then transferred to MASP2-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-MBL2 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of MBL2 and MASP2 is shown in Figure 4.
Endotoxin Level	< 1.0 EU per 1 µg (LAL method)
Concentration	Prior to lyophilization: 200 µg/ml
Availability	Shipped within 5-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.

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Human Mannose Binding Lectin (MBL) Protein (Active)

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