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Apoptosis Signal Regulating Kinase 1 (MAP3K5) Antibody
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Catalogue No: abx011152
Price:
US$427.75
(Size: 100 µl)
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Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Research Articles on Apoptosis Signal Regulating Kinase 1 (MAP3K5)
| Target | Apoptosis Signal Regulating Kinase 1 (MAP3K5) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, IF/ICC, FCM |
| Host | Mouse |
| Recommended dilutions | ELISA: 1/10000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant fragment of human MAP3K5 expressed in E. coli. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Unpurified ascites. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q99683 (UniProt, ExPASy) |
| UniProt Secondary AC | A6NIA0, B4DGB2, Q5THN3, Q99461 |
| UniProt Entry Name | M3K5_HUMAN |
| Gene Symbol | MAP3K5 |
| GeneID | 4217 |
| OMIM | 602448 |
| HGNC | 6857 |
| KEGG | hsa:4217 |
| Ensembl | ENSG00000197442 |
| String | 9606.ENSP00000351908 |
| Enzyme Commission Number | EC 2.7.11.25 |
| Molecular Weight | 55 kDa |
| Buffer | Ascitic fluid containing 0.03% sodium azide. |
| Concentration | Not determined. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |