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MAP4K1 (pS171) Antibody
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Catalogue No: abx031990
Price:
US$326.25
(Size: 80 µl)
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The c-Jun amino-terminal kinases (JNKs) /stress-activated protein kinases (SAPKs) play a crucial role in stress responses in mammalian cells. The mechanism underlying this pathway in the hematopoietic system is unclear, but it is a key in understanding the molecular basis of blood cell differentiation. We have cloned a novel protein kinase, termed hematopoietic progenitor kinase 1 (HPK1), that is expressed predominantly in hematopoietic cells, including early progenitor cells. HPK1 is related distantly to the p21 (Cdc42/Rac1) activated kinase (PAK) and yeast STE20 implicated in the mitogen-activated protein kinase (MAPK) cascade. Expression of HPK1 activates JNK1 specifically, and it elevates strongly AP-1-mediated transcriptional activity in vivo. HPK1 binds and phosphorylates MEKK1 directly, whereas JNK1 activation by HPK1 is inhibited by a dominant-negative MEKK1 or MKK4/SEK mutant. Interestingly, unlike PAK65, HPK1 does not contain the small GTPase Rac1/Cdc42-binding domain and does not bind to either Rac1 or Cdc42, suggesting that HPK1. activation is Rac1/Cdc42-independent. These results indicate that HPK1 is a novel functional activator of the JNK/SAPK signaling pathway.
Research Articles on MAP4K1 (pS171)
| Target | MAP4K1 (pS171) |
| Clonality | Polyclonal |
| Target Modification | Ser171 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S171 of human MAP4K1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q92918 (UniProt, ExPASy) |
| UniProt Entry Name | M4K1_HUMAN |
| KEGG | hsa:11184 |
| String | 9606.ENSP00000465039 |
| Molecular Weight | Calculated MW: 91.3 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse MAP4K1. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |