Mesoderm-Specific Transcript Homolog Protein (MEST) Antibody

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Catalogue No: abx005263
Price: US$333.50
(Size: 100 µl)

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Western blot analysis of lysates from Mouse brain, using MEST Antibody at 1/2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.
Mesoderm-Specific Transcript Homolog Protein (MEST) Antibody Mesoderm-Specific Transcript Homolog Protein (MEST) Antibody

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Datasheet SDS
MEST Antibody is a Rabbit Polyclonal antibody against MEST. This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

Target Mesoderm-Specific Transcript Homolog Protein (MEST)
Clonality Polyclonal
Reactivity Human, Mouse
Tested Applications ELISA, WB, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/1000 - 1/5000, IF/ICC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 146-335 of human MEST.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q5EB52 (UniProt, ExPASy)
UniProt Secondary AC B2R6S1, O14973, O15007, Q6AI49, Q92571
UniProt Entry Name MEST_HUMAN
Gene Symbol MEST
GeneID 4232
NCBI Accession NP_002393.2
KEGG hsa:4232
String 9606.ENSP00000223215
Molecular Weight Calculated MW: 39 kDa
Observed MW: 39 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Mesoderm-Specific Transcript Homolog Protein (MEST)
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