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DNA Mismatch Repair Protein Mlh1 (MLH1) Antibody
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Catalogue No: abx000639
Price:
US$217.50
(Size: 20 µl)
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MLH1 Antibody is a Rabbit Polyclonal antibody against MLH1. Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). 1.Modrich, P. (2006) J Biol Chem 281, 30305-9. 2.Seng, T.J. et al. (2008) Br J Cancer 99, 375-82. 3.Harley, I. et al. (2008) Gynecol Oncol 109, 384-7. 4.Mao, G. et al. (2008) J Biol Chem 283, 3211-6. 5.Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1. 6.Vasen, H.F. (2005) Fam Cancer 4, 219-25. 7.Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.
Research Articles on DNA Mismatch Repair Protein Mlh1 (MLH1)
| Target | DNA Mismatch Repair Protein Mlh1 (MLH1) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IF: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P40692 (UniProt, ExPASy) |
| UniProt Secondary AC | B4DI13, B4DQ11, E9PCU2 |
| UniProt Entry Name | MLH1_HUMAN |
| Gene Symbol | MLH1 |
| GeneID | 4292 |
| OMIM | 114500 |
| NCBI Accession | NP_000240.1 NM_000249.3 |
| HGNC | 7127 |
| KEGG | hsa:4292 |
| Ensembl | ENSG00000076242 |
| String | 9606.ENSP00000231790 |
| Molecular Weight | Calculated MW: 85 kDa Observed MW: 85 kDa |
| Buffer | PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |