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MAP Kinase-Interacting Serine/threonine-Protein Kinase 2 (MNK2) Antibody
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Catalogue No: abx033149
Price:
US$326.25
(Size: 80 µl)
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MAP kinase-interacting kinase 1 (Mnk1) and Mnk2, members of the Ser/Thr protein kinase family, bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2. Erk and p38 phosphorylate MNK1 and Mnk2, which stimulates their in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF-4E). Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. This ubiquitiously expressed protein appears to regulate transcription by phosphorylating eIF-4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta. Mnk2 has also been shown to interact with the C-terminal regions of eIF-4G1 and eIF-4G2.
Research Articles on MAP Kinase-Interacting Serine/threonine-Protein Kinase 2 (MNK2)
| Target | MAP Kinase-Interacting Serine/threonine-Protein Kinase 2 (MNK2) |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 70-100 amino acids from the N-terminal region of human MNK2 (MKNK2). |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q9HBH9 (UniProt, ExPASy) |
| UniProt Secondary AC | Q6GPI3, Q9HBH8, Q9UHR0, Q9Y2N6 |
| UniProt Entry Name | MKNK2_HUMAN |
| String | 9606.ENSP00000250896 |
| Molecular Weight | Calculated MW: 51.9 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Mouse and Rat MKNK2. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |