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Target | Galectin 3 (LGALS3) |
Origin | Monkey |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 97% |
Reconstitution | Reconstitute in ddH2O to a concentration up to 0.5 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Molecular Weight | Calculated MW: 29.8 kDa Observed MW (SDS-PAGE): 33 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Met1-Ile248 |
Sequence | MADNFSLHDA LSGSGNPNPQ GWPGPWGNQP AGAGGYPGAS YPGAYPGQAP PGVYPGQAPP GAYPGAPGAY SGASGVYPGP PSGAGAYPSP GQPSAPGAYP ATGPYGAPAG PLSVPYNLPL PGGVVPRMLI TILGTVKPNA NRIALDFKRR NDVAFHFNPR FNENNRRVIV CNTKLDNNWG REERQSVFPF ESGKPFKIQV LVESDHFKVA VNDAHLLQYN HRVKQLNEIS QLGISGDIDL TNASYTMI |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 100 µg/ml |
Availability | Shipped within 5-12 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |