Mouse Collagen Type VII Alpha 1 (COL7A1) Protein

REQUEST MORE INFO
Catalogue No: abx620131
Price: US$19,894.00
(Size: 20 mg)
Reviews: 0 Publications and Reviews
(0 Publications and Reviews)

Click on the image to see the image legend

SDS-PAGE analysis of expression tests of the target protein. The negative control is marked Ø. Briefly, two E. coli strains (1 - BL21 (DE3), and 2 - T7E) were induced with 1 mM IPTG at either 16 °C for 16 h, or at 37 °C for 4 h. The bacterial cells were collected by centrifugation. The supernatant (medium) was lysed in a buffer containing PBS, pH 7.5, and 10% glycerol. After centrifugation, the soluble supernatnant fraction was collected (native protein extraction - NPE). The insoluble fraction was collected and solubilized with a denaturing buffer (8 M Urea). After centrifugation, the supernatant was collected (denatured protein extraction - DPE). The NPE and DPE fractions were analyzed by SDS-PAGE. Based on these tests, the optimal expression was observed in strain 1 - BL21 (DE3) cells incubated with 1 mM IPTG at 37 °C for 4 h, NPE fraction.
Mouse Collagen Type VII Alpha 1 (COL7A1) Protein Mouse Collagen Type VII Alpha 1 (COL7A1) Protein

Available Options

* Size:

Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Mouse Collagen Type VII (COL7) Protein is a Recombinant Mouse protein expressed in E. coli.

Target Collagen Type VII Alpha 1 (COL7A1)
Origin Mouse
Expression Recombinant
Tested Applications SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O. Do not vortex.
Storage Store between 2-8 °C for short-term storage. Store between -20 and -80 °C for long-term storage. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q63870 (UniProt, ExPASy)
UniProt Entry Name CO7A1_MOUSE
Sequence Fragment 1048-1238 AA.
Sequence MGSSHHHHHHSSGLEVLFQGPGS ACS HGPVDVVFLL HATRDNAHNA EAVRRVLERL VSALGPLGPQ AAQVGLLTYS HRPSPLFPLN SSHDLGIILR KIRDIPYVDP SGNNLGTAVT TAHRYLLASN APGRRQQVPG VMVLLVDEPL RGDILSPIRE AQTSGLKVMA LSLVGADPEQ LRRLAPGTDP IQNFFAVDNG PGLDRAVSDL AVALCQAA
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.5.
Activity Not tested
Endotoxin Level 10 EU/ml
Concentration Prior to lyophilization: 1.87 mg/ml
Availability Shipped within 2-3 months.
Note This product is for research use only.

Not for human consumption, cosmetic, therapeutic or diagnostic use.
Research Articles on Collagen Type VII Alpha 1 (COL7A1)


Write a review

Continue
Tags:
(Click to show)