Mouse High Mobility Group Protein B1 (HMGB1) Protein

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Catalogue No: abx067106
Price: US$246.50
(Size: 10 µg)

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Datasheet SDS
Mouse HMGB1 is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).

Target High Mobility Group Protein B1 (HMGB1)
Origin Mouse
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 90%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-0.25 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P63158 (UniProt, ExPASy)
UniProt Secondary AC P07155, P27109, P27428
UniProt Entry Name HMGB1_MOUSE
KEGG mmu:15289
String 10090.ENSMUSP00000082682
Molecular Weight Calculated MW: 26.2 kDa
Observed MW: 33 kDa (determined by SDS-PAGE)

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Met1-Glu215
Sequence MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 50 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on High Mobility Group Protein B1 (HMGB1)
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