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Target | High Mobility Group Protein B1 (HMGB1) |
Origin | Mouse |
Expression | Recombinant |
Tested Applications | WB, SDS-PAGE |
Host | E. coli |
Conjugation | Unconjugated |
Form | Lyophilized |
Purity | > 90% |
Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-0.25 mg/ml. Do not vortex. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P63158 (UniProt, ExPASy) |
UniProt Secondary AC | P07155, P27109, P27428 |
UniProt Entry Name | HMGB1_MOUSE |
KEGG | mmu:15289 |
String | 10090.ENSMUSP00000082682 |
Molecular Weight | Calculated MW: 26.2 kDa Observed MW: 33 kDa (determined by SDS-PAGE) Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
Sequence Fragment | Met1-Glu215 |
Sequence | MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE |
Tag | N-terminal His tag |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
Activity | Not tested |
Concentration | Prior to lyophilization: 50 µg/ml |
Availability | Shipped within 5-7 working days. |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |