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Mouse Interleukin 27 (IL27) Protein
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Catalogue No: abx655897
Price:
US$812.00
(Size: 50 µg)
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Mouse Interleukin 27 (IL27) Protein is a Recombinant Mouse protein expressed in 293F cell.
Research Articles on Interleukin 27 (IL27)
| Target | Interleukin 27 (IL27) |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q8K3I6 (UniProt, ExPASy) O35228 (UniProt, ExPASy) |
| UniProt Entry Name | IL27A_MOUSE, IL27B_MOUSE |
| Gene Symbol | IL27, EBI3 |
| GeneID | 246779 50498 |
| KEGG | mmu:246779 mmu:50498 |
| Ensembl | ENSMUSG00000044701 |
| String | 10090.ENSMUSP00000054637 10090.ENSMUSP00000003274 |
| Molecular Weight | Calculated MW: 49.6 kDa Observed MW (SDS-PAGE): 60 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Phe29-Ser234 + (GGGGS)*3 + Tyr19-Pro228 |
| Sequence | FP TDPLSLQELR REFTVSLYLA RKLLSEVQGY VHSFAESRLP GVNLDLLPLG YHLPNVSLTF QAWHHLSDSE RLCFLATTLR PFPAMLGGLG TQGTWTSSER EQLWAMRLDL RDLHRHLRFQ VLAAGFKCSK EEEDKEEEEE EEEEEKKLPL GALGGPNQVS SQVSWPQLLY TYQLLHSLEL VLSRAVRDLL LLSLPRRPGS AWDS - GGGGSGGGGSGGGGS - YT ETALVALSQP RVQCHASRYP VAVDCSWTPL QAPNSTRSTS FIATYRLGVA TQQQSQPCLQ RSPQASRCTI PDVHLFSTVP YMLNVTAVHP GGASSSLLAF VAERIIKPDP PEGVRLRTAG QRLQVLWHPP ASWPFPDIFS LKYRLRYRRR GASHFRQVGP IEATTFTLRN SKPHAKYCIQ VSAQDLTDYG KPSDWSLPGQ VESAPHKP |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Activity | Not tested |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |