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Mouse Protein Wnt-10a (WNT10A) Protein (Active)
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Catalogue No: abx655981
Price:
US$681.50
(Size: 50 µg)
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Mouse Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) is an active recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Research Articles on Protein Wnt-10a (WNT10A)
| Target | Protein Wnt-10a (WNT10A) |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 80% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P70701 (UniProt, ExPASy) |
| UniProt Entry Name | WN10A_MOUSE |
| Gene Symbol | WNT10A |
| GeneID | 22409 |
| KEGG | mmu:22409 |
| String | 10090.ENSMUSP00000006718 |
| Molecular Weight | Calculated MW: 56.0 kDa Observed MW (SDS-PAGE): 56 kDa |
| Sequence Fragment | Ser108-Pro343 |
| Sequence | SSL ETRNKVPYES PIFSRGFRES AFAYAIAAAG VVHAVSNACA LGKLKACGCD ASRRGDEEAF RRKLHRLQLD ALQRGKGLSH GVPEHPAILP ASPGLQDSWE WGGCSPDVGF GERFSKDFLD SREPHRDIHA RMRLHNNRVG RQAVMENMRR KCKCHGTSGS CQLKTCWQVT PEFRTVGALL RNRFHRATLIRPHNRNGGQL EPGPAGAPSP APGTPGLRRR ASHSDLVYFE KSP |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) is a ligand for members of the frizzled family of seven transmembrane receptors. It is involved in the regulation of cell proliferation, differentiation, migration, and apoptosis. Overexpression of WNT10A has been associated with various diseases, including cancer, where it can contribute to tumorigenesis through activation of the Wnt-beta-catenin-TCF signaling pathway. LRP5 and LRP6, as co-receptors of Wnt signal, play a key role in the signal transduction process, therefore a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT10A and recombinant rat LRP5. Briefly, WNT10A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LRP5-coated microplate wells and incubated for 1 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-WNT10A polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody for 1 h at 37°C, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450/630 nm immediately. The binding activity of recombinant mouse WNT10A and recombinant rat LRP5 is shown in Figure 3. The EC50 is 0.026 µg/ml. |
| Endotoxin Level | < 1.0 EU per 1 µg (LAL method) |
| Concentration | Prior to lyophilization: 50 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |