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Mouse Sphingomyelin Phosphodiesterase (SMPD1) Protein (Active)
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Catalogue No: abx655958
Price:
US$754.00
(Size: 50 µg)
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Mouse SMPD1 is an active recombinant Mouse protein produced in a Eukaryotic expression system (293F cell).
Research Articles on Sphingomyelin Phosphodiesterase (SMPD1)
| Target | Sphingomyelin Phosphodiesterase (SMPD1) |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | 293F cell |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 95% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q04519 (UniProt, ExPASy) |
| UniProt Secondary AC | Q3UL52 |
| UniProt Entry Name | ASM_MOUSE |
| Gene Symbol | SMPD1 |
| GeneID | 20597 |
| KEGG | mmu:20597 |
| String | 10090.ENSMUSP00000042187 |
| Molecular Weight | Calculated MW: 65.1 kDa Observed MW (SDS-PAGE): 75 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. |
| Sequence Fragment | Tyr58-Leu626 |
| Sequence | YPL PSEGHSVKFS AIAPPLQSAF GWQNLTCPAC KVLFTALNHG LKKEPNVARV GSVAIKICKM LNIAPLDVCQ SAVHLFEDDV VEVWTRSVLS PSEACGLLLG SSCGHWDIFS TWNISLPSVP KPPPKPPSPP APGAPVSRVL FLTDLHWDHE YLEGTDPYCA DPLCCRRGSG WPPNSQKGAG FWGEYSKCDL PLRTLESLLK GLGPAGPFEM VYWTGDIPAH DVWQQSRQDQ LRALTTITDL VRKFLGPVPV YPAVGNHEST PVNGFPPPFI KGNQSSQWLY EAMAKAWEPW LPADALHTLR IGGFYALTPR PGLRLISLNM NFCSRENFWL LINSTDPAGQ LQWLVEELQA AENRGDKVHI IGHIPPGHCL KSWSWNYYKI IARYENTLAG QFFGHTHVDE FEIFYDEETL SRPLAVAFLA PSATTFINLN PGYRVYQIDG NYPGSSHVVL DHETYILNLT QANAAGGTPS WKRLYRARET YGLPDAMPAS WHNLVYRMRD DEQLFQTFWF LYHKGHPPSE PCGTPCRLAT LCAQLSARAD SPALCRHLMP NGSLPDANRL WSRPLL |
| Tag | N-terminal His tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose. |
| Activity | Active |
| Biological Activity | Specific activity: > 1000 pmol/min/µg |
| Endotoxin Level | < 1.0 EU per µg as determined by the LAL method. |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |