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Mouse T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein (Active)
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Catalogue No: abx655967
Price:
US$536.50
(Size: 50 µg)
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Mouse T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein is a recombinant active Mouse protein.
Research Articles on T-lymphocyte activation antigen CD80 / B7-1 (Anti-CD80)
| Target | T-lymphocyte activation antigen CD80 / B7-1 (Anti-CD80) |
| Origin | Mouse |
| Expression | Recombinant |
| Tested Applications | ELISA |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 90% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q00609 (UniProt, ExPASy) |
| Molecular Weight | Calculated MW: 53.7 kDa Observed MW (SDS-PAGE): 54 kDa |
| Sequence Fragment | Asp39-Asn246 |
| Sequence | DE QLSKSVKDKV LLPCRYNSPH EDESEDRIYW QKHDKVVLSV IAGKLKVWPE YKNRTLYDNT TYSLIILGLV LSDRGTYSCV VQKKERGTYE VKHLALVKLS IKADFSTPNI TESGNPSADT KRITCFASGG FPKPRFSWLE NGRELPGINT TISQDPESEL YTISSQLDFN TTRNHTIKCL IKYGDAHVSE DFTWEKPPED PPDSKN |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | EC50: 0.029 µg/ml A functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CD80 with recombinant human IL10. Briefly, serial dilutions of recombinant Mouse T-lymphocyte activation antigen CD80 / B7-1 (CD80) Protein were carried out in PBS, 0.01 % BSA (pH 7.4). Duplicate samples of 100 µl were transferred to microtiter wells pre-coated with IL10 and incubated for 1h at 37 °C. Wells were washed with PBST and incubated for 1h with anti-CD80 Polyclonal Antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant Mouse CD80 and recombinant human IL10 was shown in Figure 1. |
| Endotoxin Level | < 1.0 EU/µg (determined by the LAL method) |
| Concentration | Prior to lyophilization: 200 µg/ml |
| Availability | Shipped within 5-12 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |