Mouse VGF Nerve Growth Factor Inducible (VGF) Protein

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Catalogue No: abx655482
Price: US$232.00
(Size: 10 µg)

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Datasheet SDS
Mouse VGF Nerve Growth Factor Inducible (VGF) Protein is a Recombinant Mouse protein expressed in E. coli.

Target VGF Nerve Growth Factor Inducible (VGF)
Origin Mouse
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q0VGU4 (UniProt, ExPASy)
UniProt Entry Name VGF_MOUSE
Molecular Weight Calculated MW: 36.5 kDa
Observed MW (SDS-PAGE): 43 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Arg284-Arg576
Sequence RLEGSFL GGSEAGERLL QQGLAQVEAG RRQAEATRQA AAQEERLADL ASDLLLQYLL QGGARQRDLG GRELQETQQE RENEREEEAE QERRGGGEDD VGEEDEEAAE AEAEAEEAER ARQNALLFAE EEDGEAGAED KRSQEEAPGH RRKDAEGAEE GGEEDDDDEE MDPQTIDSLI ELSTKLHLPA DDVVSIIEEV EEKRKRKKNA PPEPVPPPRA APAPTHVRSP QPPPPAPARD ELPDWNEVLP PWDREEDEVF PPGPYHPFPN YIRPRTLQPP ASSRRRHFHH ALPPAR
Tag N-terminal His tag
Buffer Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-12 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on VGF Nerve Growth Factor Inducible (VGF)


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