mRNA Kit

REQUEST MORE INFO
Catalogue No: abx298030
Price: US$348.00
(Size: 24 rxns)

Orders to United States ship from United States

Reviews: 0 Publications and Reviews
(0 Publications and Reviews)
mRNA Kit

Click on the image to see the image legend

Available Options

* Size:


Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Abbexa's mRNA Kit uses oligo(dT)-conjugated magnetic beads to specifically bind to poly(A) tailed mRNA. It is suitable for isolating mRNA from purified highly intact total RNA (0.1-10 μg, RIN value ≥ 8). The isolated mRNA can be used in RT-PCR, qRT-PCR, next generation sequencing, and other applications. This kit is compatible with magnetic-rod high-throughput nucleic acid extractors.

Kit components:
Component 24 rxns 96 rxns
mRNA Beads1.3 ml 5 ml
Binding Buffer 1.3 ml 5 ml
Clean Buffer 1.3 ml 5 ml
Wash Buffer 10 ml 40 ml
RNase-free Water 1.3 ml 5 ml


Target mRNA Kit
Storage Store between 2-8 °C for up to 1 year.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use
  1. Take mRNA Beads out from 2-8 °C and equilibrate to room temperature. Mix well by vortexing.
  2. Prepare RNA sample: dilute the total RNA to 50 µl with RNase-free Water in a PCR tube.
  3. Pipette 50 µl of mRNA Beads into the RNA sample. Mix well by pipetting up and down.
  4. Heat the PCR tube for 5 minutes at 65 °C. Cool to 4 °C and place at room temperature for 5 minutes. If small bead precipitates are observed at the tube bottom during the reaction, mix well by vortexing.
  5. Place the PCR tube on a magnetic stand for 5 minutes. Carefully discard the supernatant.
  6. Remove the PCR tube from the magnetic stand. Add 200 µl of Wash Buffer and mix well by pipetting. Place on the magnetic stand for 5 minutes and carefully discard the supernatant.
  7. Remove the PCR tube from the magnetic stand. Add 50 µl of Clean Buffer and resuspend the beads by pipetting.
  8. Heat the tube for 2 minutes at 80 °C and cool to 25 °C.
  9. Add 50 µl of Binding Buffer and mix well by pipetting. Allow to stand at room temperature for 5 minutes.
  10. Place the PCR tube on the magnetic stand for 5 minutes. Discard the supernatant carefully.
  11. Remove the PCR tube from the magnetic stand. Add 200 µl of Wash Buffer and mix well by pipetting. Place on the magnetic stand for 5 minutes and discard the supernatant carefully.
  12. Remove the PCR tube from the magnetic stand. Add 12 µl of RNase-free Water and mix well by pipetting.
  13. Heat for 2 minutes at 80 °C and place the PCR tube on the magnetic stand. After the solution turns clear, pipette 10 µl of supernatant to an RNase-free PCR tube.
    Note: If the isolated mRNA will be used for NGS library preparation, fragmentation buffer can optionally be added (according to the fragmentation requirements) in the mRNA elution procedure. After high-temperature fragmentation, immediately place on the magnetic stand. After the solution turns clear, pipette the supernatant to an RNase-free PCR tube, which should be used immediately for library preparation or stored at -80 °C.
  14. Store the isolated mRNA at -80 °C.
Notes:
  • Use RNase-free PCR tubes
  • The total RNA sample should be highly intact (RIN value > 8), otherwise the mRNA information will be partially lost.
  • In order to obtain optimal experimental results, it is recommended to use the isolated mRNA immediately in follow-up experiments.
Research Articles on mRNA Kit


Write a review

Continue
Tags:
(Click to show)