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Myosin-9 (MYH9) Antibody
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Catalogue No: abx000580
Price:
US$217.50
(Size: 20 µl)
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MYH9 Antibody is a Rabbit Polyclonal antibody against MYH9. Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, MYH9, MYH10, and MYH14, which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7).
Research Articles on Myosin-9 (MYH9)
| Target | Myosin-9 (MYH9) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/1000 - 1/2000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P35579 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K6E4, O60805, Q60FE2, Q86T83 |
| UniProt Entry Name | MYH9_HUMAN |
| Gene Symbol | MYH9 |
| GeneID | 4627 |
| OMIM | 155100 |
| NCBI Accession | NP_002464.1 NM_002473.5 |
| HGNC | 7579 |
| KEGG | hsa:4627 |
| Ensembl | ENSG00000100345 |
| String | 9606.ENSP00000216181 |
| Molecular Weight | Calculated MW: 227 kDa Observed MW: 250 kDa |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |