Ni-IDA Resin

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Catalogue No: abx098109
Price: US$333.50
(Size: 5 ml)
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Ni-IDA Resin

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Datasheet SDS
Ni-IDA Resin allows rapid affinity purification of His-tagged proteins. His-tagged proteins bind to Ni2+ cations, which are immobilized on the Ni-IDA resin by 3 metal-chelating sites. After unbound proteins are washed away, the target proteins are recovered by gradient elution. It is suitable for both native and denatured protein purification.

Specifications:
  • Resin: Cross-linked 6% agarose
  • Ligand: IDA
  • Shape: Sphere
  • Pore Size: 90 µm
  • Binding Capacity: 20-40 mg/ml wet gel
  • Recommended Flow Rate: < 300 cm/h
  • Highest Resisistance of Atmospheric Pressure: 0.3 MPa
  • pH Stability: 2-14


Target Ni-IDA Resin
Storage Store at 2-8 °C (with 20% ethanol) for up to 2 years.
Buffer Note: Buffers are not included with this product.

Equilibration Buffer for soluble proteins: 50 mM sodium phosphate buffer, 300 mM NaCl, 10 mM imidazole, 10 mM Tris-HCl, pH 8.0.
Equilibration Buffer for inclusion bodies: 100 mM sodium phosphate buffer, 6 M GuHCl, 10 mM Tris-HCl, pH 8.0; or 100 mM sodium phosphate buffer, 8 M urea, 10 mM Tris-HCl, pH 8.0.
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Preparing the Ni-IDA purification column:
  1. Thoroughly resuspend the Ni-IDA resin to achieve a homogeneous suspension of the resin in 20% ethanol storage buffer.
  2. Immediately transfer the resin into a purification column. Ensure that the bottom of the column is plugged with a stopper. Close the value of the column and allow the resin to settle.
  3. Equilibrate the column with 5-10 bed volume of equilibration buffer.
Preparing samples:

To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading.

Loading samples and washing:

Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube

Elute:

Elute proteins with imidazole or low pH buffer.

Regeneration of Ni-IDA resin:

  1. Wash the column/resin with:
    1. 2 bed volume of 6 M GuHCl, 0.2 M acetic acid.
    2. 5 bed volume of deionised water.
    3. 3 bed volume of 2% SDS.
    4. 1 bed volume of 25% ethanol.
    5. 1 bed volume of 50% ethanol.
    6. 1 bed volume of 75% ethanol.
    7. 5 bed volume of 100% ethanol.
    8. 1 bed volume of 75% ethanol.
    9. 1 bed volume of 50% ethanol.
    10. 1 bed volume of 25% ethanol.
    11. 1 bed volume of deionised water.
    12. 5 bed volume of 100 mM EDTA, pH 8.0.
    13. 10 bed volume of deionised water.
    14. 5 bed volume of 100 mM NiSO4.
  2. Store the column/resin in 20% ethanol.
Research Articles on Ni-IDA Resin


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