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Rat Noggin (NOG) Protein (Active)
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Catalogue No: abx651424
Price:
US$522.00
(Size: 50 µg)
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Rat Noggin (NOG) Protein (Active) is a recombinant active Rat protein expressed in E. coli.
Research Articles on Noggin (NOG)
| Target | Noggin (NOG) |
| Origin | Rat |
| Tested Applications | WB, SDS-PAGE |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% |
| Reconstitution | To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q62809 (UniProt, ExPASy) |
| UniProt Entry Name | NOGG_RAT |
| Molecular Weight | Calculated MW: 63.6 kDa Observed MW: 64 kDa |
| Sequence Fragment | Met28-Cys144 |
| Sequence | MPS EIKGLEFSEG LAQGKKQRLS KKLRRKLQMW LWSQTFCPVL YAWNDLGSRF WARYVKVGSC FSKRSCSVPE GMVCKPSKSV HLTVLRWRCQ RRGGQRCGWI PIQYPIISEC KCSC |
| Tag | N-terminal His tag and MBP tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
| Activity | Active |
| Biological Activity | Noggin (NOG) is a signaling molecule that plays an important role in promoting somite patterning in developing embryos and is essential for cartilage morphogenesis and joint formation. It is an inhibitor of several bone morphogenetic proteins (BMPs), including BMP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat NOG and BMP4. Briefly, NOG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BMP4-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-NOG polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of NOG with BMP4 is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU/µg (LAL method) |
| Concentration | Prior to lyophilization: 400 µg/ml |
| Availability | Shipped within 5-7 working days. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |