Nuclear Factor, Erythroid Derived 2 Like 2 (NFE2L2) Cell ELISA Kit

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Catalogue No: abx595429
Price: US$572.75
(Size: 96 tests)

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Nuclear Factor, Erythroid Derived 2 Like 2 (NFE2L2) Cell ELISA Kit

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Datasheet SDS Download Manual
Nrf2 Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.

Introduction Nuclear Factor, Erythroid 2 Like 2 (NFE2L2, also known as nuclear factor erythroid 2-related factor) is a transcription factor from the basic leucine zipper class. The protein is rapidly degraded by KEAP1 and Cullin 3-mediated ubiquitination in normal conditions, but this can be prevented by oxidative stress. Once the cysteine residues in KEAP1 are oxidised, the degradation of NFE2L2 is inhibited, allowing it to translocate to the nucleus. It then binds to a Maf protein and initiates the transcription of antioxidant proteins via Antioxidant Response Element (ARE) promoters. Affected genes include sulphiredoxin, glutathione-S-transferase, and heme oxygenase. NFE2L2 is expressed in some cancers to prevent oxidative damage from neutrophils, and mutations in this gene are associated with developmental delay and immune dysregulation.
Target Nuclear Factor, Erythroid Derived 2 Like 2 (NFE2L2)
Reactivity Human, Mouse, Rat
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Storage Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity 6 months.
UniProt Primary AC Q16236 (UniProt, ExPASy)
UniProt Secondary AC B2RBU2, B4E338, E9PGJ7, Q53RW6, Q59HH2, Q96F71
UniProt Entry Name NF2L2_HUMAN
Gene Symbol NFE2L2
GeneID 4780
OMIM 600492
HGNC 7782
KEGG hsa:4780
Ensembl ENSG00000116044
String 9606.ENSP00000380252
Detection Method Colorimetric
Plate Coating Uncoated
Substrate TMB
Detection Antibody Conjugation HRP
Availability Shipped within 1-2 weeks.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
Research Articles on Nuclear Factor, Erythroid Derived 2 Like 2 (NFE2L2)
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