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p16-INK4A (pS140) Antibody
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Catalogue No: abx031883
Price:
US$326.25
(Size: 80 µl)
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p16-INK4A functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. This protein acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. The gene for this protein is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Research Articles on p16-INK4A (pS140)
| Target | p16-INK4A (pS140) |
| Clonality | Polyclonal |
| Target Modification | Ser140 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S140 of human p16-INK4A. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P42771 (UniProt, ExPASy) |
| UniProt Entry Name | CDN2A_HUMAN |
| NCBI Accession | NP_000068.1 NP_001182061.1 NP_478104.2 |
| KEGG | hsa:1029 |
| String | 9606.ENSP00000418915 |
| Molecular Weight | Calculated MW: 16.5 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |