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P21CIP1 (pT57) Antibody
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Catalogue No: abx031889
Price:
US$326.25
(Size: 80 µl)
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This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Research Articles on P21CIP1 (pT57)
| Target | P21CIP1 (pT57) |
| Clonality | Polyclonal |
| Target Modification | Thr57 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T57 of human P21CIP1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P38936 (UniProt, ExPASy) |
| UniProt Secondary AC | Q14010, Q6FI05, Q9BUT4 |
| UniProt Entry Name | CDN1A_HUMAN |
| KEGG | hsa:1026 |
| String | 9606.ENSP00000384849 |
| Molecular Weight | Calculated MW: 18.1 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |