p27Kip1 (pS10) Antibody

This product is currently in development. The lead time for this product may be several months. Please contact us at [email protected] for an updated lead time before purchasing this product.

p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

Target	p27Kip1 (pS10)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1.
Isotype	IgG
Form	Liquid
Purification	Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P46527 (UniProt, ExPASy)
UniProt Secondary AC	Q16307, Q5U0H2, Q9BUS6
UniProt Entry Name	CDN1B_HUMAN
NCBI Accession	NP_004055.1
KEGG	hsa:1027
String	9606.ENSP00000228872
Molecular Weight	Calculated MW: 22.1 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse and Hamster CDKN1B.
Availability	Please enquire.
Note	This product is for research use only.

Research Articles on p27Kip1 (pS10)

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