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p27Kip1 (pT157) Antibody
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Catalogue No: abx031892
Price:
US$326.25
(Size: 80 µl)
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p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Research Articles on p27Kip1 (pT157)
| Target | p27Kip1 (pT157) |
| Clonality | Polyclonal |
| Target Modification | Thr157 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, IHC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T157 of human p27Kip1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P46527 (UniProt, ExPASy) |
| UniProt Secondary AC | Q16307, Q5U0H2, Q9BUS6 |
| UniProt Entry Name | CDN1B_HUMAN |
| NCBI Accession | NP_004055.1 |
| KEGG | hsa:1027 |
| String | 9606.ENSP00000228872 |
| Molecular Weight | Calculated MW: 22.1 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |