Mitogen-Activated Protein Kinase 1 and 3 / ERK1/2 (MAPK1/MAPK3) Antibody

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Catalogue No: abx011157
Price: US$435.00
(Size: 100 µl)

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Datasheet SDS
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is an important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway. MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK and the transcription factor Elk-1. p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors such as U0126 and PD98059.

Target Mitogen-Activated Protein Kinase 1 and 3 / ERK1/2 (MAPK1/MAPK3)
Clonality Monoclonal
Clone S578
Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, FCM
Host Mouse
Recommended dilutions ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Purified recombinant fragment of human MAPK expressed in E. coli.
Isotype IgG2b
Form Liquid
Purification Unpurified ascites.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P27361 (UniProt, ExPASy) P28482 (UniProt, ExPASy)
GeneID 5594
Molecular Weight 42 kDa
Buffer Ascitic fluid containing 0.03% sodium azide.
Concentration Not determined.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Mitogen-Activated Protein Kinase 1 and 3 / ERK1/2 (MAPK1/MAPK3)