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p53 (pT18) Antibody
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Catalogue No: abx031899
Price:
US$326.25
(Size: 80 µl)
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Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Research Articles on p53 (pT18)
| Target | p53 (pT18) |
| Clonality | Polyclonal |
| Target Modification | Thr18 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, DB |
| Host | Rabbit |
| Recommended dilutions | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04637 (UniProt, ExPASy) |
| UniProt Entry Name | P53_HUMAN |
| KEGG | hsa:7157 |
| String | 9606.ENSP00000269305 |
| Molecular Weight | Calculated MW: 43.7 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Pig and Monkey TP53. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |