p53 (Sumoylation Site Specific) Antibody

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Catalogue No: abx031604
Price: US$326.25
(Size: 80 µl)

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Datasheet SDS
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2 and ARF-mediated nucleolar targeting.

Target p53 (Sumoylation Site Specific)
Clonality Polyclonal
Reactivity Human
Tested Applications ELISA, WB, IHC
Host Rabbit
Recommended dilutions WB: 1/2000, IHC-P: 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen KLH-conjugated synthetic peptide between 364-393 amino acids from human p53.
Isotype IgG
Form Liquid
Purification Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P04637 (UniProt, ExPASy)
UniProt Entry Name P53_HUMAN
NCBI Accession NP_000537.3 NP_001119584.1 NP_001119585.1 NP_001119586.1 NP_001119587.1 NP_001119588.1 NP_001119589.1 NP_001119590.1 NP_001263624.1 NP_001263625.1 NP_001263626.1 NP_001263627.1 NP_001263628.1 NP_00126
KEGG hsa:7157
String 9606.ENSP00000269305
Molecular Weight Calculated MW: 43.7 kDa
Buffer PBS containing 0.09% sodium azide.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on p53 (Sumoylation Site Specific)


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