p63 alpha Antibody

The p63 gene is a homologue of the p53 tumor suppressor gene. Like p53, p63 contains a transactivation (TA) domain induce the transcription of target genes, a DNA binding domain, and an oligomerization domain (OD), used to form tetramers. In contrast to p53, the p63 gene encodes for at least six major isotypes. Three isotypes (TAp63 alpha, TAp63 beta, and TAp63 gamma) contain the transactivating (TA) domain and are able to transactivate p53 report genes and induce apoptosis. In contrast, the other three isotypes (delta Np63 alpha, delta Np63 beta, delta Np63 gamma) are transcribed from an internal promoter localized within intron3, lack the TA domain, and act as dominant-negatives to suppress transactivation by both p53 and TAp63 isotypes. p63 is highly expressed in the basal cells of the epithelium significant for proper limb outgrowth and morphogenesis.4 In differentiating tissues, p63 is crucial for maintaining the stem cell identity of the basal cells, and is indispensable for correct development of the skin as well as the limb. p63-deficient mice lack all squamous epithelia and their derivatives, including hair, whiskers, teeth, as well as mammary, lacrimal, and salivary glands.Tissue specificity: Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of delta N-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.

Target	p63 alpha
Clonality	Monoclonal
Clone	I504
Reactivity	Human, Mouse, Rat, Monkey
Tested Applications	ELISA, WB, IHC
Host	Mouse
Recommended dilutions	ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Synthesized peptide corresponding to human p63a (CARRNKQQRIKEEGE).
Isotype	IgG1
Form	Liquid
Purification	Purified from ascites by Protein G chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9H3D4 (UniProt, ExPASy)
UniProt Entry Name	P63_HUMAN
Gene Symbol	TP63
GeneID	8626
Molecular Weight	Calculated MW: 51 kDa
Buffer	PBS containing 0.05% sodium azide.
Concentration	Not determined.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on p63 alpha

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