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3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1) Antibody
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Catalogue No: abx031631
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US$326.25
(Size: 80 µl)
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Datasheet SDS
Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98% identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.
Research Articles on 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1)
| Target | 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1) |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 592-624 amino acids from the C-terminal region of human PAPSS1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at 2-8°C for up to 2 weeks, or at -20°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | O43252 (UniProt, ExPASy) |
| UniProt Secondary AC | O43841, O75332, Q6IAX6, Q96FB1, Q96TF4, Q9P1P9, Q9UE98 |
| UniProt Entry Name | PAPS1_HUMAN |
| Gene Symbol | PAPSS1 |
| NCBI Accession | NP_005434.4 |
| KEGG | hsa:9061 |
| String | 9606.ENSP00000265174 |
| Molecular Weight | Calculated MW: 70.8 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |