Phosphorylated Adapter RNA Export Protein (PHAX) Antibody

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC) bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG) capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Target	Phosphorylated Adapter RNA Export Protein (PHAX)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human PHAX.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9H814 (UniProt, ExPASy)
UniProt Secondary AC	Q9H8W1
UniProt Entry Name	PHAX_HUMAN
Gene Symbol	PHAX
String	9606.ENSP00000297540
Molecular Weight	Calculated MW: 44.4 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat and Cow PHAX.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Phosphorylated Adapter RNA Export Protein (PHAX)

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