PI3KC3 (pS164) Antibody

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

Target	PI3KC3 (pS164)
Clonality	Polyclonal
Target Modification	Ser164
Modification	Phosphorylation
Reactivity	Human
Tested Applications	ELISA, DB
Host	Rabbit
Recommended dilutions	DB: 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S164 of human PI3KC3.
Isotype	IgG
Form	Liquid
Purification	Purified by protein A affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with peptides. The antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q8NEB9 (UniProt, ExPASy)
UniProt Secondary AC	Q15134
UniProt Entry Name	PK3C3_HUMAN
KEGG	hsa:5289
String	9606.ENSP00000262039
Molecular Weight	Calculated MW: 102 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Mouse, Rat and Pig PIK3C3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on PI3KC3 (pS164)

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