Pig Brain Derived Neurotrophic Factor (BDNF) Protein

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Catalogue No: abx065595
Price: US$536.50
(Size: 50 µg)

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Pig Brain Derived Neurotrophic Factor (BDNF) Protein Pig Brain Derived Neurotrophic Factor (BDNF) Protein

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Datasheet SDS
Pig BDNF Protein is a recombinant Pig protein produced in a Prokaryotic expression system (E. coli).

Target Brain Derived Neurotrophic Factor (BDNF)
Origin Pig
Expression Recombinant
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 95%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P14082 (UniProt, ExPASy)
UniProt Entry Name BDNF_PIG
Gene Symbol BDNF
GeneID 397495
KEGG ssc:397495
String 9823.ENSSSCP00000014166
Molecular Weight Calculated MW: 27.4 kDa
Observed MW (SDS-PAGE): 32 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Pro20-Arg252
Sequence P MKEANVRGQG SLAYPGVRTH GTLESVNGPK AGSRGLTSSS SSSLADTFEH VIEELLDEDQ KVRPNEENNK DADMYTSRVM LSSQVPLEPP LLFLLEEYKN YLDAANMSMR VRRHSDPARR GELSVCDSIS EWVTAADKKT AVDMSGGTVT VLEKVPVSKG QLKQYFYETK CNPMGYTKEG CRGIDKRHWN SQCRTTQSYV RALTMDSKKR IGWRFIRIDT SCVCTLTIKR GR
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose.
Activity Not tested
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Brain Derived Neurotrophic Factor (BDNF)


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