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Target | Inactivated Complement C3b (iC3b) |
Clonality | Polyclonal |
Reactivity | Pig |
Expression | Recombinant |
Tested Applications | ELISA, WB, SDS-PAGE |
Host | Rabbit |
Recommended dilutions | WB, Recombinant protein: 0.2-0.8 µg per lane, 1/64000-1/256000. WB, Endogenous lysate: 10-30 µg per lane, 1/1000-1/4000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | Recombinant protein corresponding to 747-1302 AA derived from Pig iC3b expressed in E.coli. |
Isotype | IgG |
Form | Liquid |
Purification | Purified by affinity chromatography using epitope-specific immunogen. |
Storage | Store at 4 °C for short-term storage. For long-term storage, aliquot and store at -20°C, with up to 50% glycerol. Store at a concentration between 0.5-2 mg/ml. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P01025 (UniProt, ExPASy) |
UniProt Entry Name | CO3_PIG |
Molecular Weight | Calculated MW: 65.2 kDa |
Sequence | MGSSHHHHHH SSGLEVLFQG PGSSDLDEEI IPEEDIISRS QFPESWLWTI EEFKEPDKNG ISTKTMNVFL KDSITTWEIL AVSLSDKKGI CVADPYEVVV KQDFFIDLRL PYSVVRNEQV EIRAILYNYR EAEDLKVRVE LLYNPAFCSL ATAKKRHQQT LTVPAKSSVP VPYIIVPLKT GLQEVEVKAA VYNHFISDGV KKTLKVVPEG MRVNKTVVTR TLDPEHKGQQ GVQREEIPPA DLSDQVPDTE SETKILLQGT PVAQMVEDAI DGDRLKHLIQ TPSGCGEQNM IGMTPTVIAV HYLDSTEQWE KFGLEKRQEA LELIKKGYTQ QLAFRQKNSA FAAFQDRLSS TLLTAYVVKV FAMAANLIAI DSQVLCGAVK WLILEKQKPD GVFEENGPVI HQEMIGGFKN TEEKDVSLTA FVLIALQEAK DICEPQVNSL LRSINKARDF LADYYLELKR PYTVAIAGYA LALSDKLDEP FLNKLLSTAK ERNRWEEPGQ KLHNVEATSY ALLALLVVKD FDSVPPIVRW LNEQRYYGGG YGSTQATFMV FQALAQYQKD VPDHKDLNLD VSIHLPSRS |
Buffer | PBS, pH 7.4 |
Specificity | Recognises Pig iC3b Protein in Indirect ELISA and Western blot. |
Concentration | Antibody: 3.53 mg/ml Protein: 1.4 mg/ml |
Availability | Shipped within 8-11 weeks. |
Note | This product is for research use only. This product is shipped with ice packs. Not for human consumption, cosmetic, therapeutic or diagnostic use. |