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Pig Interferon Beta (IFNb) Protein (Active)
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Catalogue No: abx655692
Price:
US$5,394.00
(Size: 1 mg)
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Pig Interferon Beta (IFNb) Protein (Active) is an Active Recombinant Pig protein.
Research Articles on Interferon Beta (IFNb)
| Target | Interferon Beta (IFNb) |
| Origin | Pig |
| Expression | Recombinant |
| Tested Applications | WB, SDS-PAGE |
| Host | E. coli |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 98% |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Molecular Weight | Calculated MW: 49.62 kDa Observed MW (SDS-PAGE): 50 kDa |
| Sequence Fragment | Met22-Asn186 |
| Sequence | MSYDVLRYQ QRSSNLACQK LLGQLPGTPQ YCLEDRMNFE VPEEIMQPPQ FQKEDAVLII HEMLQQIFGI LRRNFSSTGW NETVIKTILV ELDGQMDDLE TILEEIMEEE NFPRGDMTIL HLKKYYLSIL QYLKSKEYRS CAWTVVQVEI LRNFSFLNRL TDYLRN |
| Tag | N-terminal His tag and GST tag |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl and 5% Trehalose. |
| Activity | Active |
| Biological Activity | Interferon Beta (IFNb) is a member of type I interferons (IFNs) family which is a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNb proteins are produced in large quantities by fibroblasts. They have antiviral activity that is involved mainly in innate immune response. Two types of IFNb have been described, IFNb1 (IFNB1) and IFNb3 (IFNB3). IFNb1 is used as a treatment for multiple sclerosis as it reduces the relapse rate. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNb, thus a binding ELISA assay was conducted to detect the interaction of recombinant Pig IFNb and recombinant Pig IFNa/bR1. Briefly, IFNb were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to IFNa/bR1-coated microtiter wells and incubated for 2 h at 37 °C .Wells were washed with PBST and incubated for 1 h with anti-IFNb polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 min at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of IFNb and IFNa/bR1 is shown in Figure 4. |
| Endotoxin Level | < 1.0 EU per 1 μg (LAL method) |
| Concentration | Prior to lyophilization: 50 µg/ml |
| Availability | Shipped within 1-2 months. |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |