1-Phosphatidylinositol 3-Phosphate 5-Kinase (PIKFYVE) Antibody

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Catalogue No: abx005129
Price: US$406.00
(Size: 100 µl)

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Western blot analysis of lysates from Mouse lung, using PIKFYVE Antibody at 1/500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 180s.
1-Phosphatidylinositol 3-Phosphate 5-Kinase (PIKFYVE) Antibody

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Datasheet SDS
PIKFYVE Antibody is a Rabbit Polyclonal antibody against PIKFYVE. Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Target 1-Phosphatidylinositol 3-Phosphate 5-Kinase (PIKFYVE)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 269-545 of human PIKFYVE.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q9Y2I7 (UniProt, ExPASy)
UniProt Secondary AC Q08AR7, Q08AR8, Q53ST3, Q53T36, Q8N5H0, Q8NB67
UniProt Entry Name FYV1_HUMAN
Gene Symbol PIKFYVE
GeneID 200576
OMIM 121850
NCBI Accession NP_055855.2
KEGG hsa:200576
String 9606.ENSP00000264380
Molecular Weight Calculated MW: 237 kDa
Observed MW: 250 kDa
Buffer PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on 1-Phosphatidylinositol 3-Phosphate 5-Kinase (PIKFYVE)
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