Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) Antibody

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Catalogue No: abx012448
Price: US$58.00
(Size: 10 µg)

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Western blot analysis of lysates from mouse brain, using PKA alpha/beta CAT (Phospho-Thr197) Antibody. The lane on the right is blocked with the phosphopeptide.
Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) Antibody Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) Antibody Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197) Antibody

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Datasheet SDS
Rabbit polyclonal antibody against PKA alpha/beta CAT protein when phosphorylated at Thr197. Immunogen region is Internal. Specificity is as follows for the reactive species: H:T198, M:T198, R:T198.

Target Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197)
Clonality Polyclonal
Target Modification Thr197
Modification Phosphorylation
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions WB: 1/500 - 1/3000, IHC: 1/50 - 1/100, IF/ICC: 1/100 - 1/500, ELISA: 1/10000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKA alpha/beta CAT around the phosphorylation site of threonine 197 (T-W-TP-L-C).
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using the non-phosphopeptide corresponding to the phosphorylation site.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P17612 (UniProt, ExPASy) P22694 (UniProt, ExPASy)
UniProt Entry Name KAPCA_HUMAN, KAPCB_HUMAN
Sequence VKGRTWTLCGTPEY
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
Specificity Detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197.
Concentration 1 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Protein Kinase, cAMP Dependent Catalytic Alpha/Beta Phospho-Thr197 (PRKACA/PRKACB pT197)
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