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DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) Antibody
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Catalogue No: abx001719
Price:
US$203.00
(Size: 20 µl)
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POLR2A Antibody is a Rabbit Polyclonal antibody against POLR2A. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Research Articles on DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A)
| Target | DNA-Directed RNA Polymerase II Subunit RPB1 (POLR2A) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | WB, IF/ICC, IP |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/200, IP: 0.5-4 µg/200-400 µg whole cell extracts. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POLR2A. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P24928 (UniProt, ExPASy) |
| UniProt Secondary AC | A6NN93, B9EH88, Q6NX41 |
| UniProt Entry Name | RPB1_HUMAN |
| Gene Symbol | POLR2A |
| GeneID | 5430 |
| OMIM | 180660 |
| NCBI Accession | NP_000928.1 |
| HGNC | 9187 |
| KEGG | hsa:5430 |
| Ensembl | ENSG00000181222 |
| String | 9606.ENSP00000480158 |
| Molecular Weight | Calculated MW: 217 kDa Observed MW: 210-250 kDa |
| Buffer | PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |