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Palmitoyl-Protein Thioesterase 1 (PPT1) Antibody
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Catalogue No: abx031626
Price:
US$326.25
(Size: 80 µl)
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Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8 and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.
Research Articles on Palmitoyl-Protein Thioesterase 1 (PPT1)
| Target | Palmitoyl-Protein Thioesterase 1 (PPT1) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 32-63 amino acids from the N-terminal region of human PPT1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P50897 (UniProt, ExPASy) |
| UniProt Secondary AC | B4DY24, Q6FGQ4 |
| UniProt Entry Name | PPT1_HUMAN |
| KEGG | hsa:5538 |
| String | 9606.ENSP00000394863 |
| Molecular Weight | Calculated MW: 34.2 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Cow and Monkey PPT1. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |