Palmitoyl-Protein Thioesterase 1 (PPT1) Antibody

Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many thioesterases and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8 and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

Target	Palmitoyl-Protein Thioesterase 1 (PPT1)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 269-300 amino acids from the C-terminal region of human PPT1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P50897 (UniProt, ExPASy)
UniProt Secondary AC	B4DY24, Q6FGQ4
UniProt Entry Name	PPT1_HUMAN
KEGG	hsa:5538
String	9606.ENSP00000394863
Molecular Weight	Calculated MW: 34.2 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Monkey PPT1.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Palmitoyl-Protein Thioesterase 1 (PPT1)

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