Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody

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Catalogue No: abx001289
Price: US$232.00
(Size: 20 µl)
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Western blot analysis of lysates from wild type (WT) and PKA RIIα (PRKAR2A)/PKR2 knockout (KO) HeLa cells, using [KO Validated] PKA RIIα (PRKAR2A) Antibody at 1/1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A) Antibody

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Datasheet SDS
PRKAR2A Antibody is a Rabbit Polyclonal antibody against PRKAR2A. The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory ® and two catalytic © subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.

Target Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/1000 - 1/2000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RIIα (PRKAR2A/PKR2).
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P13861 (UniProt, ExPASy)
UniProt Secondary AC Q16823, Q9BUB1
UniProt Entry Name KAP2_HUMAN
Gene Symbol PRKAR2A
GeneID 5576
NCBI Accession NP_004148.1
KEGG hsa:5576
String 9606.ENSP00000265563
Molecular Weight Calculated MW: 46 kDa
Observed MW: 46 kDa
Buffer PBS, pH 7.3, containing 0.09% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on Protein Kinase cAMP-Dependent Type II Regulatory Subunit Alpha (PRKAR2A)


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