Human Protein Kinase R (PKR) Protein (Active)

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Catalogue No: abx651433
Price: US$928.00
(Size: 50 µg)
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SDS-PAGE analysis of recombinant Human PKR.
Human Protein Kinase R (PKR) Protein (Active) Human Protein Kinase R (PKR) Protein (Active) Human Protein Kinase R (PKR) Protein (Active) Human Protein Kinase R (PKR) Protein (Active)

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Datasheet SDS
Human Protein Kinase R (PKR) Protein (Active) is a recombinant active Human protein expressed in E. coli.

Target Protein Kinase R (PKR)
Origin Human
Tested Applications WB, SDS-PAGE
Host E. coli
Conjugation Unconjugated
Form Lyophilized
Purity > 80%
Reconstitution Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
Storage Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P19525 (UniProt, ExPASy)
UniProt Secondary AC A8K3P0, D6W584, E9PC80, Q52M43, Q7Z6F6, Q9UIR4
UniProt Entry Name E2AK2_HUMAN
KEGG hsa:5610
String 9606.ENSP00000233057
Molecular Weight Calculated MW: 35.8 kDa
Observed MW: 32 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Sequence Fragment Ser224-Ile502
Sequence SSSLLMN GLRNNQRKAK RSLAPRFDLP DMKETKYTVD KRFGMDFKEI ELIGSGGFGQ VFKAKHRIDG KTYVIKRVKY NNEKAEREVK ALAKLDHVNI VHYNGCWDGF DYDPETSDDS LESSDYDPEN SKNSSRSKTK CLFIQMEFCD KGTLEQWIEK RRGEKLDKVL ALELFEQITK GVDYIHSKKL IHRDLKPSNI FLVDTKQVKI GDFGLVTSLK NDGKRTRSKG TLRYMSPEQI SSQDYGKEVD LYALGLILAE LLHVCDTAFE TSKFFTDLRD GI
Tag N-terminal His tag
Buffer Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Activity Active
Biological Activity Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused by viruses. PKR can also be activated by the protein PACT or by heparain. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CDK1-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-PKR polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of PKR and CDK1 is shown in Figure 4.
Endotoxin Level < 1.0 EU/µg (LAL method)
Concentration Prior to lyophilization: 200 µg/ml
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Protein Kinase R (PKR)
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