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Target | Proto-Oncogene c-Fos (FOS) |
Clonality | Monoclonal |
Clone | O369 |
Reactivity | Human, Mouse, Rat |
Tested Applications | ELISA, WB, IF/ICC, IP |
Host | Rabbit |
Recommended dilutions | ELISA: 1/20000, WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | Unconjugated |
Immunogen | Synthetic peptide derived from the internal region of human c-FOS. |
Epitope Region | Leu197-Leu210 |
Isotype | IgG |
Form | Liquid |
Purification | Purified from rabbit antiserum by proprietary techniques. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P01100 (UniProt, ExPASy) P01101 (UniProt, ExPASy) P12841 (UniProt, ExPASy) |
UniProt Secondary AC | A8K4E2, B4DQ65, P18849, Q4FDN1 |
UniProt Entry Name | FOS_HUMAN, FOS_MOUSE, FOS_RAT |
Gene Symbol | FOS |
GeneID | 2353 14281 314322 |
KEGG | hsa:2353 mmu:14281 rno:314322 |
String | 9606.ENSP00000306245 |
Buffer | 20 mM Tris-HCl, pH 8.0, containing 10 mg/ml BSA and 0.05% NaN3. |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |
Directions for use | For WB, it is recommended to use 1X Tris Buffered Saline (TBS) with 0.1% Triton X-100 as the wash buffer; 1X TBS with 0.1% Triton X-100 and 5% nonfat dry milk as the blocking buffer; and to incubate the membrane with the antibody diluted in blocking buffer for 2 hours at room temperature. |