Proteasome Inhibitor Subunit 1 (PSMF1) Antibody

REQUEST MORE INFO
Catalogue No: abx004253
Price: US$217.50
(Size: 20 µl)
Reviews: 0 Publications and Reviews
(0 Publications and Reviews)

Click on the image to see the image legend

Western blot analysis of various lysates, using PSMF1 Antibody at 1/500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1/10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
Proteasome Inhibitor Subunit 1 (PSMF1) Antibody Proteasome Inhibitor Subunit 1 (PSMF1) Antibody

Available Options

* Size:



Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
PSMF1 Antibody is a Rabbit Polyclonal antibody against PSMF1. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Target Proteasome Inhibitor Subunit 1 (PSMF1)
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1 µg/ml, WB: 1/100 - 1/500, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-271 of human PSMF1.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q92530 (UniProt, ExPASy)
UniProt Secondary AC A0AVQ9, D3DVW3, Q9H4I1
UniProt Entry Name PSMF1_HUMAN
Gene Symbol PSMF1
GeneID 9491
NCBI Accession NP_006805.2
KEGG hsa:9491
String 9606.ENSP00000338039
Molecular Weight Calculated MW: 30 kDa
Observed MW: 30 kDa
Buffer PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Concentration > 0.2 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Proteasome Inhibitor Subunit 1 (PSMF1)
Tags:
(Click to show)