Human Pulmonary Surfactant-Associated Protein D (SFTPD) ELISA Development Kit
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Price:
US$609.00
(Size: 5 × 96 tests)
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Pulmonary Surfactant-Associated Protein D (SFTPD) ELISA Development Kit for use in Sandwich ELISA assay development.
This ELISA Development Kit contains:
Component | 5 × 96 tests | 15 × 96 tests |
Pretreated 96-well ELISA Plate | 5 | 15 |
Capture Antibody | 120 µl | 350 µl |
Biotin-Conjugated Detection Antibody | 120 µl | 350 µl |
HRP-Conjugate | 120 µl | 350 µl |
Standard | 1 vial | 3 vials |
Please note that quantities and concentrations may change between different batches.
It is recommended to use this ELISA Development Kit with abx471002 ELISA Development Support Kit (Sandwich Method); alternatively, the following solutions can be prepared separately:
- Coating Buffer: 1X Citrate-Buffered Saline
- Blocking Buffer: 1X PBS containing 0.5-3% BSA
- Wash Buffer: 3% Tris
- Standard and Sample Diluent Buffer: 1X PBS containing 0.5-3% BSA
- Antibody and HRP-Conjugate Diluent Buffer: 1X PBS containing 0.5-3% BSA
- Stop Solution: 5% Sulfuric Acid
Target |
Pulmonary Surfactant-Associated Protein D (SFTPD) |
Reactivity |
Human |
Tested Applications |
ELISA |
Recommended dilutions |
Capture Antibody: 1/500 - 1/1000, Biotin-conjugated Detection Antibody: 1/500 - 1/1000, HRP-Conjugate: 1/500 - 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
Reconstitution |
Reconstitute the standard with 1 ml of Standard Diluent, then serially dilute as required. |
Storage |
Aliquot and store at -20°C in the dark. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC |
P35247 (UniProt,
ExPASy)
|
UniProt Secondary AC |
Q5T0M3, Q6FH08, Q86YK9, Q8TCD8, Q9UCJ2, Q9UCJ3 |
UniProt Entry Name |
SFTPD_HUMAN |
Gene Symbol |
SFTPD |
GeneID |
6441
|
OMIM |
178635
|
KEGG |
hsa:6441
|
String |
9606.ENSP00000361366
|
Test Range |
1.56 ng/ml - 100 ng/ml |
Detection Method |
Colormetric |
Assay Type |
Sandwich |
Sample Type |
Serum and plasma. |
Availability |
Shipped within 5-10 working days. |
Note |
This product is for research use only. |
Directions for use |
Bring all components to room temperature (18-25°C) and briefly spin or centrifuge the vials before use. Working solutions should be prepared and used immediately.
Recommended Procedure: - Dilute the Capture Antibody to working concentration using Coating Buffer. Immediately coat the 96-well plate with diluted Capture Antibody (100 µl per well). Seal the plate and incubate at 2-8 °C overnight.
- Remove the liquid from each well. Do not wash. Block the plate with Blocking Buffer (200 µl per well) at 37 °C for 1 hour.
- Remove the liquid from each well. Do not wash. Either proceed with the following steps immediately or dry the plate at 37 °C for 30 minutes, then store at -20 °C with dessicant for up to 6 months.
- Add 100 µl of standards or sample into the appropriate wells. Cover with a plate sealer and incubate at 37 °C for 1.5 hours.
- Remove the liquid from each well. Do not wash. Add appropriately diluted Biotin-Conjugated Detection Antibody (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 1 hour.
- Remove the liquid from each well. Wash with Wash Buffer (350 µl per well) and allow to soak for 1-2 min. Remove the liquid by inverting and tapping the plate on to absorbent paper. Repeat the wash process 3 times.
- Add appropriately diluted HRP-Conjugate (100 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 30 min.
- Repeat the wash process in Step 6, for a total of 5 times.
- Add Substrate Solution (90 µl per well). Cover the plate with a new plate sealer and incubate at 37 °C for 15-30 min. Keep the plate in the dark and avoid exposure to light.
- Add Stop Solution (50 µl per well). Tap the side of the plate to ensure thorough mixing.
- Measure the absorbance immediately using a microplate reader set at 450 nm.
|
Research Articles on Pulmonary Surfactant-Associated Protein D (SFTPD)