qPCR Extraction Control Red

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Catalogue No: abx461005
Price: US$5,234.50
(Size: 2000 rxns)
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qPCR Extraction Control Red

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Datasheet SDS
qPCR Extraction Control Red. This product comprises of cells that contain an internal control DNA sequence, with no known homology to any organism and a specific primer/probe control mix for PCR detection. It is used to reduce the chance of obtaining false negative results following DNA extraction.

Target qPCR Extraction Control Red
Tested Applications PCR
Storage Store at -20 °C. Avoid repeated freeze/thaw cycles.
Validity Up to 12 months.
Buffer The exact formulation is proprietary.
Kit Components Kit Components:

Component Volume
Internal Control DNA
25X Control Mix 25 µl
Availability Shipped within 3-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use All reactions should be carried out at room temperature unless stated otherwise.

Extraction Steps:
  1. Thaw and spin down all tubes before opening.
  2. Vortex the tube containing the Internal Control DNA to ensure complete mixing.
  3. Add 5 µl Internal Control DNA per sample to the lysis buffer (not provided). Ensure the mixture is well homogenised before loading onto samples (see next step).
  4. Follow the manufacturer's protocol for sample DNA extraction.
  5. The remaining Internal Control DNA mixture can be stored at 4 °C.
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Recommended reagent volumes per 25 µl qPCR mix:

Component Volume
2X PCR Master Mix (not provided) 12.5 µl
Target Probe/Primer Mix (not provided) N/A
Sample DNA from extraction step (not provided) N/A
25X Control Mix 1 µl
Total Volume 25 µl

Assay Setup:

Step Number of Cycles Temperature Time per Cycle
Polymerase Activation 1 cycle 95 °C 10 min
Denaturation30-40 cycles 95 °C 15 seconds
Annealing/Extension60 °C 30-60 seconds

Notes:
  • The fluorescence signal for the DNA Internal Control can be observed at 670 nm.
  • The qPCR conditions above are suitable for amplicons of up to 200 bp, however they can be varied to suit different qPCR mixes and machine-specific protocols.
  • Ct values of the Internal Control may vary due to elution volumes of nucleic acid and reagents used.
  • The optimal conditions will vary from reaction to reaction and are dependent on the system used.
  • Each parameter needs to be adjusted by the end user and some optimization may be required.
Research Articles on qPCR Extraction Control Red


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